Welcome! In this newsletter we will be talking about cGMP level Karyotyping.
What is Karyotyping?
Karyotyping is a biological laboratory test that examines the number and structure of chromosomes in cells. When working with induced pluripotent stem cells (iPSCs), karyotyping is essential to ensure that the cells have maintained genetic stability. Because iPSCs are used in research and potential therapies, it is important to confirm that their chromosomes remain normal and free of abnormalities.
How we can help
At Allele, we offer a complete karyotyping preparation service for iPSC lines, making it easy to get your samples ready for analysis. The cells are cultured for four to five days in strictly monitored conditions, including controlled temperature, humidity, and nutrient supply, to promote healthy growth and maintain chromosomal integrity. They are then carefully packaged in flasks filled completely with media to maintain cell health during transit. Careful preparation is crucial for obtaining accurate karyotyping results, since even minor mistakes in timing, technique, or packaging can compromise the quality of the samples.
Why choose Allele?
All preparation is conducted in full compliance with cGMP standards, ensuring that every step adheres to strict, standardized protocols and undergoes comprehensive quality control. This rigorous framework not only guarantees the highest level of consistency and safety but also helps reduce overall costs by eliminating the need for expensive in-house facilities, specialized staff training, and ongoing validation efforts.
Additional note: beyond karyotyping, we highly recommend full genome sequencing of iPSCs as upstream materials for clinical development and at critical points during derivation towards therapeutic cells. Ask us for help with data collection and processing.
Stay tuned for our next newsletter where we talk about RNA sequencing (RNAseq).
