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	<title>AlleleBlog &#187; promoter-FP</title>
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	<link>http://allelebiotech.com/blogs</link>
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		<title>Fluorescent Protein-Based Assay Development</title>
		<link>http://allelebiotech.com/blogs/2010/03/fluorescent-protein-based-assay-development/</link>
		<comments>http://allelebiotech.com/blogs/2010/03/fluorescent-protein-based-assay-development/#comments</comments>
		<pubDate>Wed, 10 Mar 2010 17:28:49 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[Fluorescent proteins]]></category>
		<category><![CDATA[Open Forum]]></category>
		<category><![CDATA[assay development]]></category>
		<category><![CDATA[biosensor]]></category>
		<category><![CDATA[cell based assays]]></category>
		<category><![CDATA[chemical screening]]></category>
		<category><![CDATA[circular permutation]]></category>
		<category><![CDATA[fluorescent protein]]></category>
		<category><![CDATA[FRET]]></category>
		<category><![CDATA[GFP]]></category>
		<category><![CDATA[mTFP1]]></category>
		<category><![CDATA[mWasabi]]></category>
		<category><![CDATA[promoter-FP]]></category>
		<category><![CDATA[RNAi screening]]></category>

		<guid isPermaLink="false">http://allelebiotech.com/blogs/?p=783</guid>
		<description><![CDATA[Genes encoding FPs alone or as fusions to a protein of interest may be introduced to cells by a number of different methods, including simple plasmid transfection or viral transduction. Once expressed, FPs are easily detected with standard fluorescence microscopy equipment.]]></description>
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		<title>Monitoring the Undifferentiated Stage of Stem Cells—the Pluripotency Markers</title>
		<link>http://allelebiotech.com/blogs/2010/01/monitoring-the-undifferentiated-stage-of-stem-cells%e2%80%94the-pluripotency-markers/</link>
		<comments>http://allelebiotech.com/blogs/2010/01/monitoring-the-undifferentiated-stage-of-stem-cells%e2%80%94the-pluripotency-markers/#comments</comments>
		<pubDate>Thu, 28 Jan 2010 21:16:46 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[iPS and other stem cells]]></category>
		<category><![CDATA[differentiation]]></category>
		<category><![CDATA[fluorescent protein]]></category>
		<category><![CDATA[iPS]]></category>
		<category><![CDATA[iPS lentivirus]]></category>
		<category><![CDATA[iPSC]]></category>
		<category><![CDATA[iPSCs]]></category>
		<category><![CDATA[lentivirus]]></category>
		<category><![CDATA[pluripotency reporter]]></category>
		<category><![CDATA[promoter]]></category>
		<category><![CDATA[promoter-FP]]></category>
		<category><![CDATA[stem cells]]></category>
		<category><![CDATA[undifferentiation stage]]></category>

		<guid isPermaLink="false">http://allelebiotech.com/blogs/?p=744</guid>
		<description><![CDATA[There have been a number of publications using murine Oct-4, Nanog, and Rex-1 promoter driven fluorescent proteins as markers for pluripotency tests [1-3].  Allele Biotech provides, under its iPS product line, packaged and validated lentiviral particles that would insert these 3 promoter-FP reporters into the stem cells.  Although currently these promoters are of mouse sequences, their use in human stem cells have been reported.]]></description>
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