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	<title>AlleleBlog &#187; gene knockdown</title>
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		<title>Choosing siRNA, shRNA, and miRNA for Gene Silencing</title>
		<link>http://allelebiotech.com/blogs/2010/02/choosing-sirna-shrna-and-mirna-for-gene-silencing/</link>
		<comments>http://allelebiotech.com/blogs/2010/02/choosing-sirna-shrna-and-mirna-for-gene-silencing/#comments</comments>
		<pubDate>Wed, 03 Feb 2010 21:27:17 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[RNAi patent landscape]]></category>
		<category><![CDATA[Viruses and cells]]></category>
		<category><![CDATA[gene knockdown]]></category>
		<category><![CDATA[gene silencing]]></category>
		<category><![CDATA[lentivirus packaging]]></category>
		<category><![CDATA[miRNA]]></category>
		<category><![CDATA[packaging systems]]></category>
		<category><![CDATA[RNAi]]></category>
		<category><![CDATA[shRNA]]></category>
		<category><![CDATA[shRNA packaging]]></category>
		<category><![CDATA[siRNA]]></category>
		<category><![CDATA[virus packaging service]]></category>

		<guid isPermaLink="false">http://allelebiotech.com/blogs/?p=756</guid>
		<description><![CDATA[What is the most effective format for RNAi in mammalian cells?  It is also difficult to rank the efficiency of shRNA versus miRNA from published data, partly due to different results from different experimental systems. ]]></description>
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		<title>RNAi Design, Validation and Target Screening</title>
		<link>http://allelebiotech.com/blogs/2009/11/rnai-design-validation-and-target-screening/</link>
		<comments>http://allelebiotech.com/blogs/2009/11/rnai-design-validation-and-target-screening/#comments</comments>
		<pubDate>Wed, 18 Nov 2009 19:31:27 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[Fluorescent proteins]]></category>
		<category><![CDATA[RNAi patent landscape]]></category>
		<category><![CDATA[cell based assays]]></category>
		<category><![CDATA[DNA damage repair]]></category>
		<category><![CDATA[gene knockdown]]></category>
		<category><![CDATA[gene silencing]]></category>
		<category><![CDATA[lentiviral vector]]></category>
		<category><![CDATA[Pol III promoter]]></category>
		<category><![CDATA[RNAi]]></category>
		<category><![CDATA[RNAi design]]></category>
		<category><![CDATA[RNAi screening]]></category>
		<category><![CDATA[RNAi service]]></category>
		<category><![CDATA[RNAi targets]]></category>
		<category><![CDATA[shRNA]]></category>
		<category><![CDATA[siRNA]]></category>
		<category><![CDATA[Synthetic lethal]]></category>

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		<description><![CDATA[RNAi screening has been conducted to identify correlations between gene functions and cellular phenotypes such as synthetic lethality among DNA damage signaling and repair pathway factors. Successfully performing high throughput RNAi screenings requires capabilities of efficient RNAi design, viral packaging, fluorescent proteins, and advanced cell culture and analysis techniques. ]]></description>
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